Whole Blood
Begin with a whole-blood sample containing the complete cellular background.
Applications
Circulating Tumor Cells
Enrich rare EpCAM-expressing circulating tumor cells from whole blood for downstream cellular and molecular research.
SAMPLE
Whole Blood
TARGET
EpCAM-Expressing Cells
INPUT RANGE
1 mL – 50 mL*
RARE CELL ENRICHMENT
When the target is rare, enrichment becomes critical.
Circulating tumor cells (CTCs) are intact tumor-derived cells found in the bloodstream. Because they can occur at very low abundance relative to surrounding blood cells, enrichment is an important upstream step before CTC detection and characterization.1
Unlike cell-free nucleic acids, intact CTCs retain cellular morphology and can provide access to both cellular and molecular information. Enriched CTC populations can support research workflows including immunocytochemistry, nucleic acid analysis, PCR, sequencing, and cell-based studies.1
The Revolution CTC Enrichment Kit (Epithelial Origin) uses anti-EpCAM antibodies and proprietary magnetic bead technology to enrich intact, viable EpCAM-expressing epithelial-derived CTCs from human whole blood.
RARE CELL TARGET
Focus the population
Enrich EpCAM-expressing cells from the much larger cellular background present in whole blood.
INTACT CELLS
Preserve morphology
Gentle processing is designed to preserve CTC viability and cellular morphology.
DOWNSTREAM FLEXIBILITY
More research options
Carry enriched cells into cellular, imaging, and molecular research workflows.
THE CTC CHALLENGE
Find the rare cells before you characterize them.
Whole blood contains an overwhelming background of erythrocytes, leukocytes, platelets, and other cellular material. Against that background, CTCs can represent an extremely rare target population.
Enrichment reduces background and concentrates the targeted cell population, creating a more practical starting point for downstream cellular and molecular characterization.
RARE CELL ENRICHMENT
Reduce the background. Concentrate the target. Preserve the cells.
RARE TARGET
Enrich before downstream analysis
EpCAM-TARGETED ENRICHMENT
From whole blood to an enriched CTC population.
The Revolution CTC workflow combines antibody-based targeting with magnetic enrichment to concentrate EpCAM-expressing epithelial-origin cells from whole blood.
The goal is not simply to detect a marker. It is to recover an enriched population of intact cells that can be carried into downstream research workflows.
TARGETING SCOPE
This is an EpCAM-dependent enrichment workflow designed for
epithelial-origin CTCs displaying the EpCAM epitope.
EpCAM
Anti-EpCAM antibodies provide label-dependent targeting of EpCAM-expressing epithelial-origin cells.
Magnetic Capture
Proprietary magnetic bead technology enriches the targeted cell population.
Intact Cells
Move the enriched population into downstream cellular or molecular research.
RESEARCH USE ONLY
Revolution CTC Enrichment Kit (Epithelial Origin)
The Revolution CTC Enrichment Kit uses proprietary magnetic bead technology and anti-EpCAM antibodies to enrich intact, viable epithelial-derived circulating tumor cells from human whole blood.
Enrich CTCs displaying the EpCAM epitope
Process 1 mL through 50 mL in a single enrichment*
Inquire with nRichDX about larger sample volumes up to 100 mL*
Designed to preserve cell viability and morphology
Supports downstream cellular and molecular research
For Research Use Only.
Not for use in diagnostic procedures.
*Current product specifications. Contact nRichDX regarding
requirements outside the standard workflow.
REVOLUTION CTC ENRICHMENT
EpCAM-targeted enrichment from whole blood
INPUT RANGE
1–50 mL*
DOWNSTREAM APPLICATIONS
Intact cells keep more research options open.
Enriched CTC populations can support both cellular and molecular investigation after the upstream enrichment step.
Immunocytochemistry
Evaluate enriched cells using imaging and immunocytochemical research workflows.
Nucleic Acids
Extract DNA or RNA from enriched cells for PCR and other molecular workflows.
Sequencing
Investigate genomic or transcriptomic features within enriched cell populations.
Viable Cells
Gentle processing is designed to preserve viable cells for downstream cell-based research.
A BROADER LIQUID BIOPSY VIEW
One blood sample can provide access to multiple analyte classes.
Whole blood contains both plasma-based and cellular analytes. Plasma can provide access to circulating cell-free nucleic acids, while the cellular fraction can be used for CTC enrichment.
nRichDX has demonstrated coordinated Revolution workflows in which different fractions from the same whole-blood sample are used for CTC enrichment and cfDNA or cfTNA extraction.
WHOLE BLOOD
One starting sample
PLASMA FRACTION
cfDNA / cfTNA
CELLULAR FRACTION
CTC Enrichment
CTC SCIENTIFIC RESOURCES
Go deeper into CTC enrichment on the Revolution platform.
Explore the Revolution CTC Enrichment Kit, product information, performance data, and related liquid biopsy resources for Research Use Only workflows.
EpCAM Enrichment
Whole-Blood Workflows
Cellular + Molecular Analysis
REFERENCES
Literature cited
- Alix-Panabières C, Pantel K. “Circulating Tumor Cells: Liquid Biopsy of Cancer.” Clinical Chemistry. 2013;59(1):110–118.
- nRichDX. Revolution CTC Enrichment Kit (Epithelial Origin) Instructions for Use. For Research Use Only. Not for use in diagnostic procedures.