Applications

Extraction from Urine

Build a recovery-focused liquid biopsy workflow around an abundant, non-invasive biological sample.

SAMPLE Urine

ANALYTES cfDNA + cfRNA / cfTNA

KIT RANGE 1 mL – 50 mL

Applications cfDNA cfRNA | cfTNA Urine Preanalytical Urine CTC Exosomes Infectious Disease

APPLICATIONS Extraction from Urine

URINE LIQUID BIOPSY

A high-volume opportunity for liquid biopsy.

Urine is a non-invasive biological sample that contains multiple molecular components relevant to liquid biopsy, including DNA, RNA, extracellular vesicles, and cellular material.¹ Urine-based liquid biopsy has been investigated in both urological and non-urological cancers.^1,2

Unlike blood collection, urine can be collected non-invasively and in comparatively large quantities, making repeat sampling and larger-volume molecular workflows possible.^1,2

For laboratories developing urine-based molecular assays, sample preparation determines how much of that available specimen can be processed and how much molecular material is successfully carried forward into downstream analysis.

NON-INVASIVE SAMPLE

Accessible biofluid

Urine can be collected without a blood draw or invasive tissue procedure.

VOLUME FLEXIBILITY

More available sample

Urine provides flexibility for evaluating larger liquid biopsy inputs.

MULTIPLE COMPARTMENTS

More than cfDNA

Urine contains cell-free and cell-associated molecular material.

HIGH-VOLUME EXTRACTION

Larger urine inputs should not require a fragmented workflow.

As specimen input increases, conventional extraction approaches can require additional handling, transfers, processing units, pooling, or concentration steps.

Each additional manual operation can increase workflow complexity and create another opportunity for sample loss or variability. The Revolution workflow is designed to consolidate larger urine inputs into a more streamlined sample-preparation process.

50 mL WORKFLOW A consolidated approach to large-volume urine sample preparation.

Comparison of Revolution sample preparation and column-based workflows for 50 mL urine

WHOLE URINE

One urine sample contains more than one molecular compartment.

Urine contains cell-free nucleic acids in the supernatant as well as cellular material that can contribute DNA and RNA to downstream molecular analysis.³

In a 2026 nRichDX study, a whole-urine single-extraction workflow was compared with fractionated pellet and supernatant workflows under comparable extraction conditions.³

7.7%–25.1% higher mean DNA yield Whole urine vs. paired pellet + supernatant sum across study runs

RNA yield preserved Relative to supernatant-only extraction under study conditions

KRAS G12V improved analytical recovery Whole urine compared with either fraction alone

View the 2026 Poster

RECOVERY STARTS UPSTREAM

What enters the assay defines what can be detected.

For low-abundance molecular targets, downstream analysis begins with the material successfully recovered during sample preparation. Sample input, analyte recovery, and transfer determine the molecular starting point available to the assay.

DESIGN AROUND THE SAMPLE Use the available specimen intentionally, then carry recovered molecular material forward.

START WITH Available Urine

RECOVER Molecular Material

CARRY FORWARD Usable Analyte

REVOLUTION URINE EXTRACTION

Match the chemistry and sample volume to the analyte.

Current Revolution cfDNA and cfTNA kit configurations support urine sample inputs from 1 mL through 50 mL.

Select the analyte and sample-volume range that fits the workflow, then use the Revolution Kit Selector to identify the appropriate current configuration.

01 STARTING INPUT 1 mL

50 MAX RANGE Up to 50 mL

Use the Kit Selector

CHOOSE BY ANALYTE + VOLUME Four current urine-compatible configurations.

cfDNA | 1 mL – 20 mL

Revolution cfDNA Max 20 Kit

Cell-free DNA extraction from urine across sample inputs from 1 mL through 20 mL.

View in Kit Selector

cfDNA | >20 mL – 50 mL

Revolution cfDNA Max 50 Kit

Cell-free DNA extraction from larger urine sample inputs greater than 20 mL and up to 50 mL.

View in Kit Selector

cfTNA / cfRNA | 1 mL – 20 mL

Revolution cfTNA Max 20 Kit

Cell-free total nucleic acid extraction, including cfRNA, from urine inputs from 1 mL through 20 mL.

View in Kit Selector

cfTNA / cfRNA | >20 mL – 50 mL

Revolution cfTNA Max 50 Kit

Cell-free total nucleic acid extraction from larger urine inputs greater than 20 mL and up to 50 mL.

View in Kit Selector

Comparative Evaluation of Whole Urine and Pellet-Based Extraction Workflows white paper

WHITE PAPER · 2026 Urine Liquid Biopsy

FEATURED WHITE PAPER

Comparative Evaluation of Whole Urine and Pellet-Based Extraction Workflows for Urine Liquid Biopsy

Explore a deeper discussion of whole-urine single extraction versus fractionated processing and the impact of simplifying upstream urine sample preparation.

The white paper expands on the workflow considerations behind processing whole urine and the opportunity to reduce unnecessary fractionation, transfers, and manual handling.

Find the White Paper View the Supporting Poster

URINE LIQUID BIOPSY RESOURCES

Go deeper into the urine liquid biopsy workflow.

Explore posters, publications, application data, and collaborative studies spanning high-volume urine extraction, cfDNA, cfRNA, preservation, and downstream molecular analysis.

Whole-Urine Data High-Volume Workflows Posters & Publications

View Reference Materials Request a Quote

REFERENCES

Literature cited

Oshi M, et al. “Urine as a Source of Liquid Biopsy for Cancer.” Cancers. 2021;13(11):2652.
Jain S, Lin SY, Song W, Su YH. “Urine-Based Liquid Biopsy for Nonurological Cancers.” Genetic Testing and Molecular Biomarkers. 2019;23(4):277–283.
Jafari N, Saenz J, Hernandez C, Cedeno D, Van Dieren C, Saidian M. “Comparative Evaluation of Whole Urine and Pellet-Based Extraction Workflows for Urine Liquid Biopsy.” Presented at the 10th Liquid Biopsy for Precision Oncology Summit, February 2026.