PRODUCTS · cfDNA REFERENCE STANDARD

RUO PN 409000

cfDNA Reference Standard

Determine the efficiency of cfDNA extraction from plasma or urine samples.

A convenient spike-and-recovery reference material designed to help quantify extraction recovery by comparing the amount added to a sample with the amount recovered after purification.

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LIST PRICE $490.00 USD

FORMAT 5 Single-Use Tubes

CONCENTRATION 1 ng/µL

Format 5 × Single-Use Tubes

Volume 1 mL Per Tube

Concentration 1 ng/µL in TE Buffer

Expected mnDNA 150 bp

cfDNA REFERENCE STANDARD PN 409000 · RUO

MEASURE EXTRACTION RECOVERY

A straightforward way to evaluate how much cfDNA your workflow actually recovers.

Spike a known amount of reference material into the sample, perform the extraction, then compare the recovered amount with the known input to calculate percent recovery.

Convenient

Five individual thaw-and-use tubes simplify repeat testing.

Control

Determine the efficiency of your cfDNA extraction method.

Standard

Supports spike-and-recovery cfDNA extraction experiments.

SPIKE · EXTRACT · MEASURE

Turn recovery into a measurable workflow variable.

The reference standard provides a known input so extraction recovery can be calculated rather than assumed.

Prepare the Sample

Begin with the plasma or urine sample and your selected extraction workflow.

Spike Known Input

Add a known amount of the cfDNA Reference Standard to the sample.

Perform Extraction

Process the spiked sample using the extraction method being evaluated.

Calculate Recovery

Compare the spike-in amount with the recovered amount to determine percent recovery.

REFERENCE MATERIAL CHARACTERISTICS

Mononucleosomal DNA with defined molecular features.

The reference standard contains mononucleosomal DNA derived from the NCI-H441 lung cancer cell line and includes KRAS G12V and TP53 Arg158Leu variants.

The material is deproteinized. Fragment sizes are nucleosome-defined, but histones are removed during purification.

Primary Expected Fragment 150 bp

Dinucleosome 340 bp

Trinucleosome 560 bp

Source NCI-H441

cfDNA Reference Standard fragment tracing — REFERENCE PROFILE
Representative fragment tracing of the cfDNA Reference Standard.

EXPECTED RESULTS

Reference material profile and expected fragment distribution.

The current nRichDX reference-standard documentation includes gel and table data illustrating the expected material profile.

cfDNA Reference Standard expected results gel and table — EXPECTED RESULTS
Expected results for the cfDNA Reference Standard.

PCR ASSAY INFORMATION

KRAS qPCR reference information.

The current product page provides KRAS G12V probe and primer sequence information, preparation guidance, and cycling conditions for researchers using the standard.

KRAS qPCR SEQUENCES Reference Assay Components

Type	Description	Quantity	Sequence
KRAS Probe	MGB Probe	60 nmoles	/56-FAM/CTGTATCGTCAAGGCACT/3MGBEc/
KRAS G12V	Forward Primer	40–50 nmoles	AAACTTGTGGTAGTTGGAGCAGT
KRAS G12V	Reverse Primer	40–50 nmoles	CATATTCGTCCACAAAATGATTCTG

PREPARATION KRAS Probe · 5 µM

Description	500 µL	1 mL	2 mL	5 mL
KRAS Probe (10 µM)	250 µL	500 µL	1000 µL	2500 µL
10 mM Tris, 1 mM EDTA, pH 8.0	250 µL	500 µL	1000 µL	2500 µL

PREPARATION KRAS G12V Primer Pool · 3.5 µM

Description	500 µL	1 mL	2 mL
KRAS G12V Primer (6.25 µM)	280 µL	560 µL	1120 µL
10 mM Tris, 1 mM EDTA, pH 8.0	220 µL	440 µL	880 µL

CYCLING CONDITIONS FOR THE ASSAY

PCR cycling reference

Current cycling conditions provided with the reference-standard assay information.

cfDNA Reference Standard PCR assay cycling conditions

PRODUCT RESOURCES

Documentation & ordering

Access the current instructions for use or purchase the reference standard online.

INSTRUCTIONS FOR USE cfDNA Reference Standard IFU ONLINE PURCHASE Purchase cfDNA Reference Standard · $490.00 USD

REFERENCE

Spike-and-recovery workflow literature

Lampignano R, et al. Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work Flows. Clinical Chemistry. 2020;66(1):149–160. doi: 10.1373/clinchem.2019.306837. PMID: 31628139.

IMPORTANT PRODUCT LABELING

For Research Use Only.

The nRichDX cfDNA Reference Standard is for Research Use Only. Not for use in diagnostic procedures.

MEASURE WHAT YOUR WORKFLOW RECOVERS

Use a known input to evaluate cfDNA extraction efficiency.

Spike. Extract. Measure. Quantify recovery.

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