REVOLUTION PRO™ SYSTEM AND KITS

Revolution Pro System and Kits

Automated liquid biopsy extraction for your laboratory workflow.

Revolution Pro automates extraction for cfDNA, cfRNA, and cfTNA workflows using the appropriate reagents and protocol. Choose Revolution Pro/ES for fully automated processing, or Revolution Pro + Hamilton STAR for a workflow that includes manual rack transfer before automated wash and elution.

Find Your Kit Schedule a Live Demonstration View Specifications
80%–95% extraction efficiency Up to 24 samples per batch cfDNA, cfRNA, and cfTNA
nRichDX Revolution Pro automated sample preparation system nRichDX nRicher Cartridge

Automated recovery-focused sample preparation
1–100 mL per extraction; up to 50 mL for cell-free applications.

01 80%–95% Extraction Efficiency
02 1–100 mL Single-Extraction Range*
03 24 Samples per Batch
04 ~2 Hours Processing Time

* Up to 50 mL for cell-free applications.

CHOOSE THE RIGHT PATH TO AUTOMATION

Choose your automation configuration.

Choose Revolution Pro with the Revolution Elution Station (ES) for the fully automated Revolution Pro/ES configuration, or Revolution Pro with Hamilton STAR for automated extraction followed by manual rack transfer to automated wash and elution.

FULLY AUTOMATEDIVD
nRichDX Revolution Pro ES integrated automation system

Revolution Pro/ES

Integrated automation designed to minimize operator intervention from sample preparation through purified analyte.

  1. Revolution ProExtraction
  2. Revolution ESWash + elution
WorkflowFully automated processing with the Revolution ES accessory for wash and elution.
Revolution Elution Station (ES)

Revolution Elution Station (ES)

The ES accessory performs washing, drying, and final analyte elution after Revolution Pro processing. This configuration uses the ES Reagent Kit for those steps.

AutomationFully Automated
Designed ForSample preparation through purified analyte
Explore Reagent Options
HIGHLY AUTOMATEDResearch Use Only
nRichDX Revolution Pro + Hamilton STAR automation platform

Revolution Pro + Hamilton STAR

Scalable automation designed to support high-throughput batch processing.

  1. Revolution ProExtraction
  2. OperatorRack transfer
  3. Hamilton STARWash + elution
WorkflowAutomated extraction on Revolution Pro, followed by a manual rack transfer to Hamilton STAR for automated wash and elution.
Hamilton STAR automation platform

Hamilton STAR workflow

Hamilton STAR performs automated wash and elution after an operator transfers the rack from Revolution Pro. Reagent requirements depend on the Hamilton configuration.

AutomationHighly automated
Designed ForHigh-throughput batch processing
Explore the Hamilton Partnership
nRichDX + HAMILTON

Revolution Pro extraction.
Hamilton STAR wash and elution.

The nRichDX and Hamilton partnership connects Revolution Pro sample preparation with Hamilton STAR automation, giving laboratories another configuration for their liquid biopsy extraction workflow.

A clearly defined workflow

Revolution Pro performs extraction. An operator transfers the rack to Hamilton STAR for automated wash and elution.

Evaluate the workflow for your laboratory

Discuss your target analyte, sample type, input volume, and processing requirements with our team to assess the appropriate configuration and reagents.

Explore the supporting science

Review available application materials for the methods, sample types, and results relevant to your workflow.

Discuss Your Hamilton Workflow Explore Scientific References
nRichDXHamilton
  1. Revolution ProExtraction
  2. OperatorManual rack transfer
  3. Hamilton STARAutomated wash and elution
REVOLUTION PRO AT A GLANCE Review the specifications for your laboratory.

From single-pass sample handling through automated throughput, the specifications below define the operating range of Revolution Pro.

REVOLUTION PRO FEATURES

Revolution Pro specifications.

Review Revolution Pro specifications for sample input, throughput, workflow flexibility, and continuous operation.

nRichDX Revolution Pro
Extraction Efficiency80%–95%
Samples per Batch24
Throughput per Shift120
Hands-On Time10 min
SYSTEM SPECIFICATIONSRevolution Pro
Input, capacity, and operation
01Extraction, Input & Elution
Revolution Pro/ESFully automated processing with the Revolution ES accessory for wash and elution
Pro + Hamilton STARHighly automated: Pro extraction, manual rack transfer, and automated wash and elution on Hamilton STAR
Extraction Efficiency80%–95%
Sample Volumes (single extraction)1 mL–100 mLUp to 50 mL for cell-free applications
Elution Options25 µL to 100 µL across the sample volume range
02Analytes, Samples & Batch Setup
AnalytescfDNA, cfRNA, and cfTNA, with the appropriate kit and protocol
Sample TypesPlasma, Cell-free Urine, Whole Urine, Whole Blood, CSF, Peritoneal Fluid, Other Body Fluids
Samples per Batch24
Sample FlexibilityExtract from multiple sample volumes and sample types in the same batch for a shared analyte workflow
03Processing Time & Capacity
Processing Time~2 hours
Hands-On Time10 minutes per batch
Throughput per Shift120 samples
High-Throughput SupportcfDNA Base Kit: two reagent sets, each supporting up to 240 extractions at 5 mL input (up to 480 total). Each cfTNA or cfRNA supplemental kit supports up to 240 extractions at 5 mL input with the base kit. ES Reagent Kit: 240 extractions/elutions at 100 µL elution volume.
04Loading & Continuous Operation
Workflow EfficiencySemi-random access sample loading for uninterrupted workflow
Never-Dry System™Dual reagent system designed to support continuous operation
Application ModesProgrammed application modes
REVOLUTION PRO KIT SELECTOR

Select reagents for your Revolution Pro workflow.

Choose your target analyte and automation configuration to explore the reagent options for each workflow.

SYSTEMRevolution Pro

Start with the cfDNA Base Kit. Add the appropriate supplement for cfTNA or cfRNA. The ES Reagent Kit supplies wash and elution reagents for Revolution ES.

Revolution Pro cfDNA Reagent Kit, open box
Open box
Revolution Pro cfDNA Reagent Kit, closed box
Closed box
BASE KIT

Revolution Pro cfDNA Reagent Kit

Core reagents for automated cfDNA extraction. Reagent volumes adjust to the selected sample input.

System / dependency
Revolution Pro
Sample input
1–50 mL liquid biopsy samples
Capacity
Two reagent sets, each up to 240 extractions at 5 mL input. Up to 480 total at 5 mL.
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Revolution Pro cfTNA Supplemental Reagent Kit, open box
Open box
Revolution Pro cfTNA Supplemental Reagent Kit, closed box
Closed box
SUPPLEMENT · DNA + RNA

Revolution Pro cfTNA Supplemental Reagent Kit

Workflow-specific components for isolating both cell-free DNA and RNA.

System / dependency
Requires the Revolution Pro cfDNA Base Kit
Role in the workflow
Analyte-specific supplement to the base kit
Capacity
Up to 240 extractions at 5 mL input, with the base kit.
Request a Quote
Revolution Pro cfRNA Supplemental Reagent Kit, open box
Open box
Revolution Pro cfRNA Supplemental Reagent Kit, closed box
Closed box
SUPPLEMENT · RNA

Revolution Pro cfRNA Supplemental Reagent Kit

Components for cell-free RNA isolation, including DNase treatment.

System / dependency
Requires the Revolution Pro cfDNA Base Kit
Role in the workflow
Analyte-specific supplement to the base kit
Capacity
Up to 240 extractions at 5 mL input, with the base kit.
Request a Quote
Revolution ES Reagent Kit, open box
Open box
Revolution ES Reagent Kit, closed box
Closed box
WASH & ELUTION

Revolution ES Reagent Kit

Reagents for washing, drying, and final analyte elution on Revolution ES after Pro processing.

System / dependency
Revolution ES, following Revolution Pro
Elution volume
25–100 µL
Capacity
240 extractions/elutions at 100 µL elution volume.
Request a Quote

Looking for Revolution Plus kits or CTC enrichment? Explore Revolution Plus System and Kits.

SHARED RECOVERY FOUNDATION

The nRicher Cartridge recovery foundation.

The nRicher Cartridge is the recovery component used in Revolution sample preparation. Select the kit and protocol for your target analyte and sample, then choose the automation configuration for subsequent wash and elution.

01
Sample Type Flexibility

Blood, plasma, urine, CSF, peritoneal fluid, and other biofluids.

02
Broad Analyte Compatibility

cfDNA, cfRNA, and cfTNA workflows, with the appropriate kit and protocol.

nRichDX nRicher Cartridge nRicher Cartridge The shared recovery component of the Revolution platform.
03
80%–95% Extraction Efficiency

Extraction efficiency as specified for the supported workflow. Performance depends on the application and operating conditions.

04
1 mL–100 mL

A broad sample volume range in a single extraction.*

* Up to 50 mL for cell-free applications.

FROM ASSAY DEVELOPMENT TO COMMERCIAL DEPLOYMENT

Recovery for your assay.
Automation for your laboratory.

Revolution Pro supports sample-input evaluation, workflow standardization, and automated laboratory processing. Use measured assay performance and operational requirements to select the workflow.

THE DIFFERENCE Optimize for assay performance rather than for the limitations of extraction technology.
Revolution Pro brings these workflow capabilities together:
01
Extraction efficiency, 80%–95%

Evaluate recovery alongside assay performance to determine the appropriate sample input.

02
Single-pass processing

Process 1 mL to 100 mL in a single extraction, with up to 50 mL for cell-free applications. Evaluate the supported range for the selected workflow.

03
Recovery across sample inputs

Evaluate recovery across the selected sample-volume range under controlled, comparable conditions.

04
Broad analyte compatibility

Cell-free DNA, cell-free RNA, and total cell-free nucleic acid workflows, with the appropriate kit and protocol.

05
Broad sample compatibility

Including whole blood, plasma, urine, cerebrospinal fluid, peritoneal fluid, and other clinically relevant body fluids.

06
A fully automated, high-throughput workflow

Use Revolution Pro with the Revolution ES accessory for fully automated sample-to-elution processing. Validate the selected workflow for its intended application.

Use Revolution Pro to evaluate sample input and assay performance, then standardize the selected extraction workflow.
FROM RECOVERY TO ROUTINE OPERATION

Plan your daily extraction workflow.

Revolution Pro is designed to reduce hands-on burden while supporting mixed sample inputs, high-throughput reagent capacity, and continuity across the laboratory workflow.

01 10 Minutes Hands-on time per batch
02 120 Samples Throughput per shift
03 Up to 480 Extractions, depending on kit and input volume
04 Mixed Inputs Multiple sample volumes and sample types in the same batch for a shared analyte workflow
Semi-Random Access Sample loading designed for uninterrupted workflow.
Never-Dry System™ Dual reagent system designed for continuous operation.
Programmed Application Modes Programmed modes within the Revolution Pro workflow.
BUILT FOR LOW-ABUNDANCE MOLECULAR APPLICATIONS

Applications for low-abundance molecular targets.

Revolution Pro is designed around workflows where the quantity and quality of molecular input can constrain downstream analysis.

01
EARLY CANCER DETECTION

MCED / SCED

Support sample-input evaluation for Stage I and Stage II workflows where circulating tumor-derived material may be limited.

02
MINIMAL RESIDUAL DISEASE

MRD

Enable larger-volume recovery workflows for applications where residual disease signal can be extremely low.

03
MOLECULAR MONITORING

Therapy Monitoring & Molecular Profiling

Support molecular profiling workflows using cfDNA, cfRNA, or cfTNA, with the appropriate kit and protocol.

04
RESEARCH TRANSLATION

Translational Research

Standardize recovery-focused sample preparation across variable sample types, volumes, and automation requirements.

What enters the assay matters to downstream performance. Better upstream recovery preserves more opportunity for downstream analysis.
BROAD MOLECULAR COMPATIBILITY

Explore analytes and sample types.

Revolution Pro supports liquid biopsy workflows across multiple analytes and biologically relevant sample types.

cfDNACell-free DNA cfRNACell-free RNA cfTNATotal cell-free nucleic acid
SUPPORTED SAMPLE TYPES
PlasmaCell-free UrineWhole UrineWhole BloodCSFPeritoneal FluidOther Body Fluids
SCIENTIFIC & TECHNICAL RESOURCES

Explore the science behind your workflow.

Review supporting evidence, sample-input guidance, and the Revolution platform to inform your next step.

Reference Materials

Explore publications, posters, and technical resources supporting liquid biopsy sample preparation.

Explore Reference Materials

Sensitivity Ladder™

Explore the complete framework for evaluating sample input and assay performance.

Explore the Framework

Revolution Platform

Understand the shared recovery foundation across Revolution systems and workflows.

View the Platform
READY TO OPTIMIZE YOUR ASSAY PERFORMANCE?

Discuss your Revolution Pro workflow.

Tell us your target analyte, sample input, and laboratory requirements. We can help you evaluate the system configuration and reagents for your workflow.

Schedule a live Revolution Pro demonstration. Discuss input volumes and reagent requirements. Evaluate the plasma input that meets your assay’s performance requirements.
nRichDX Revolution Pro
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