PRODUCTS · CIRCULATING TUMOR CELLS

RUO PN 407000

Revolution CTC Enrichment Kit (Epithelial Origin)

Enrich intact CTCs displaying the EpCAM epitope from whole blood.

The Revolution CTC Enrichment Kit uses proprietary magnetic bead technology and anti-EpCAM antibodies to enrich intact, viable epithelial-derived circulating tumor cells using the nRichDX Revolution System.

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Whole Blood Starting sample

1–50 mL* Single extraction

Intact, Viable CTCs Downstream study

INPUT RANGE 1–50 mL*

KIT CONTENT 12 nRicher Cartridges

Starting Sample Whole Blood

Input Volume 1–50 mL*

Enrichment Anti-EpCAM · Magnetic Beads

Labeling Research Use Only

REVOLUTION CTC ENRICHMENT KIT PN 407000

PRODUCT OVERVIEW

Designed to enrich CTCs while preserving the cells you want to study.

Label-dependent enrichment targets epithelial-derived CTCs displaying the EpCAM epitope while supporting downstream cellular and molecular workflows.

Gentle Processing

Preserves CTC viability and morphology and supports downstream cell culture.

Broad Input Range

Efficiently enrich CTCs from as little as 1 mL up to 50 mL in a single extraction.

Simplified Workflow

Streamlined processing with fewer workflow steps and shorter processing time.

For larger sample volumes up to 100 mL per extraction, contact nRichDX.

LABEL-DEPENDENT CTC ENRICHMENT

A focused workflow from whole blood to enriched CTCs.

The Revolution CTC workflow combines EpCAM-targeted magnetic enrichment with the Revolution cartridge-based sample preparation architecture.

Whole Blood

Begin with whole-blood-derived material across the supported input range.

Target EpCAM

Anti-EpCAM antibodies provide label-dependent targeting of epithelial-origin CTCs.

Magnetic Enrichment

Proprietary magnetic bead technology enriches the targeted CTC population.

Downstream Analysis

Recover intact, viable cells for downstream cellular or molecular analysis.

WHAT'S IN THE KIT

One kit. Twelve cartridge-based CTC isolations.

Each Revolution CTC Enrichment Kit includes reagents and 12 × 50 mL nRicher Cartridges for label-dependent CTC isolation using the Revolution System, which is sold separately.

12 50 mL nRicher Cartridges included

KIT CONFIGURATION Reagents + 12 × 50 mL nRicher Cartridges

REAGENT CAPACITY Up to 12 isolations from samples up to 10 mL per cartridge

REQUIRED HARDWARE nRichDX Revolution System · sold separately

SUPPLEMENTAL REAGENTS Revolution CTC Reagent Kit · PN 407600

REAGENT-ONLY OPTION

RUO PN 407600

Revolution CTC Reagent Kit

Supplemental reagents for complete utilization of unused nRicher Cartridges from the Revolution CTC Enrichment Kit.

The Revolution CTC Reagent Kit does not include nRicher Cartridges. Reagent volumes are equivalent to those supplied with the CTC Enrichment Kit and are adequate for 12 CTC isolations of up to 10 mL of sample per nRicher Cartridge.

For Research Use Only. Not for use in diagnostic procedures.

View Revolution CTC Reagent Kit details

PERFORMANCE DATA

CTC recovery demonstrated across replicate enrichments.

Recovery was evaluated using MCF-7 cells spiked into human whole blood and processed with the Revolution CTC Enrichment Kit.

CTC percent recovery data — FIGURE 1
Percent recovery of 100 MCF-7 cells spiked into 10 mL human whole blood using the Revolution CTC Enrichment Kit (Epithelial Origin), replicated in separate CTC enrichments over three days.

IMMUNOFLUORESCENT MICROSCOPY

Representative single cells and cell clusters after enrichment.

Differential protein expression is shown using EpCAM, CK8, and DAPI immunofluorescence staining.

Immunofluorescent microscopy of isolated single cells — FIGURE 2 · SINGLE CELLS
Differential protein expression of EpCAM (green) and CK8 (orange) displayed by immunofluorescence staining of isolated cells. DAPI (blue). 800× magnification.

Immunofluorescent microscopy of isolated cell clusters — FIGURE 3 · CELL CLUSTERS
Differential protein expression of EpCAM (green) and CK8 (orange) displayed by immunofluorescence staining of isolated cells. DAPI (blue). 600× magnification.

Figures 2 and 3 images were taken using the Zeiss LSM 900 with Airyscan Confocal Microscope.

GET MORE FROM THE SAME BLOOD SAMPLE

CTCs can be part of a broader multi-analyte workflow.

Revolution kits may be used in tandem to enrich CTCs and isolate circulating cell-free total nucleic acid, cfDNA, and cfRNA from the same whole-blood sample.

This enables researchers to evaluate cellular and cell-free analytes within the same Revolution sample preparation framework.

View the Cancer Genomics Consortium 2023 poster

Overview of CTC enrichment and cfDNA and cfRNA extraction using Revolution Kits — Overview of enrichment of CTCs and extraction of cfDNA and cfRNA using Revolution Kits.

TapeStation traces from RNA extracted after CTC enrichment

REVOLUTION KITS WORK TOGETHER

Enrich cells first. Continue into molecular analysis.

TapeStation traces on the current product page demonstrate cfRNA extracted using the Revolution cfTNA Max 20 Kit from MCF-7 cells first isolated with the Revolution CTC Enrichment Kit.

FIGURE 4 The Revolution cfTNA Kit was used to extract cfRNA from 10³, 10⁴ and 10⁶ MCF-7 cells spiked into 10 mL whole blood and captured using the Revolution CTC Enrichment Kit.

PRODUCT RESOURCES

Documentation

Access the current product instructions and safety documentation.

INSTRUCTIONS FOR USE Revolution CTC Enrichment Kit (Epithelial Origin) IFU SAFETY DOCUMENTATION Safety Data Sheets (SDS)

IMPORTANT PRODUCT LABELING

For Research Use Only.

The Revolution CTC Enrichment Kit (Epithelial Origin) and Revolution CTC Reagent Kit are not labeled for IVD use. For Research Use Only. Not for use in diagnostic procedures.

DISCUSS YOUR CTC WORKFLOW

Talk with nRichDX about CTC enrichment and your sample-volume requirements.

EpCAM-targeted enrichment. Whole blood. Flexible input volumes.

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